Product Specifications:

Product Code DU

   The Product: Des(1-3) insulin-like growth factor-I, [Des(1-3)IGF-I]
   is a 67 amino acid analog of human IGF-I lacking the N-terminal
   tripeptide Gly-Pro-Glu.  Des(1-3) IGF-I is more potent than IGF-I in
   vitro and in vivo.  This increased potency is due to reduced
   binding of Des(1-3)IGF-I to most of the IGF binding proteins
   produced by cells.  These binding proteins normally inhibit the
   biological actions of IGF-I. The truncated peptide has been found in brain
   and other tissues.

   Source: Des(1-3)IGF-I is produced in E. coli using a
   patented expression system.  Several chromatography purification
   steps are employed to yield the Receptor Grade product.

   Purity: >95% (as determined by HPLC and N-terminal sequence
   analysis)

   Molecular Weight: 7365 daltons - confirmed by Mass
   Spectrometry

   N-terminal sequence analysis of recombinant product: 5
   residues  >95% single sequence

   Biological Activity: (stimulation of protein synthesis in rat L6
   myoblasts)  ED50 <10 ng/ml

   Endotoxin (LAL): <0.1 EU/ug

   Stability: at least 2 years at 2-4°C (lyophilised)

   Appearance: White Powder

   State: Dried from 0.1 M acetic acid: under dry nitrogen at slight
   vacuum (-25kPa)

   Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M
   acetic acid), aliquot and store at -20°C.  For lower dilutions (e.g.
   <1mg/ml) inclusion of a carrier protein is recommended.  Avoid
   freeze-thaw cycles.


This is a GroPep specification. ibt is authorized distributor of GroPep.

Rev. 2013-02

 Datasheet for Human Des(1-3)-IGF-I, recombinant protein (Receptor Grade)