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Insulin-like Growth Factors (IGF´s), Biotinylated IGF´s and Analogs, Biotinylated Insulin
Des(1-6) IGF-II, Biotinylated (Biotin-Des(1-6) IGF-II)
Des(1-6) Insulin-like Growth Factor-II, Biotinylated Synonyms: Des(1-6) Insulin-like Growth Factor-2, Biotinylated; Biotin-Des(1-6) IGF-2
Product Specifications Biotin-Des(1-6) IGF-II:
Biotin-Des(1-6) IGF-II is prepared by a selective biotinylation procedure. As the unbiotinylated analog it has onyl very weak affinity to IGF-binding proteins. Binding to recpetors is not substantially affected. Due to its strongly reduced binding affinity to the IGFBP´s, Biotin - Des (1-6) IGF-II may be used for detection of IGF-II receptors on western blots, in binding studies or in immunohistochemistry.
Examples of applications of biotinylated IGF's can be seen in application note 4.
Formulation: Lyophilised from a solution of 0.5 ml TBS, pH 7.4, containing
10 µg of the biotinylated product . A preservative and a
stabilising protein of non-mammalian origin are added to ensure stability
of the reconstituted product.
Recommended dilution: 1: 100 for western ligand blotting. Tested with
our western-ligand blotting kit.
Storage: Store refrigerated upon arrival. The product is stable at 4 °C for
at least one year. Reconstitute with 0.5 ml water and store aliquots frozen at - 20 °C. Avoid repeated freeze-thawing cycles.
Rev. 2013-02
Datasheet for Des(1-6) IGF-II, Biotinylated (Biotin-Des(1-6) IGF-II)