Product Specifications Chicken IGF-I:

The Product: Chicken IGF-I is a polypeptide comprising 70 amino acids, eight of which differ from the amino acid sequence of human IGF-I. Chicken IGF-I has been shown to stimulate growth in many cell types, including rat L6 myoblasts and chicken embryo fibroblasts, hepatocytes,adipocytes and myoblasts.

Link to Sequence:
Source: Chicken IGF-I is produced recombinantly in E. coli using a patented expression system. The chicken IGF-I molecule is correctly folded and several chromatography purification steps are employed to yield Receptor Grade chicken IGF-I.

Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)

Molecular Weight: 7738 daltons - confirmed by Mass Spectrometry

N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence.

Biological Activity: (stimulation of protein synthesis in rat L6 myoblasts)  ED50  <30 ng/ml

Endotoxin (LAL): <0.1 EU/ug

Storage/Stability: At least 2 years at 2-4° C (lyophilised)

Appearance: White Powder

State: Dried from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa)

Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C.  For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended.  Avoid freeze-thaw cycles.

Reference:Ongabesan et al:BMPs and BMPRs in chicken ovary and the effects of BMP-4 and –7 on granulosa cell proliferation and progesterone production in vitro. Am J Physiol Endocrinol Metab (July 29, 2003). 10.1152/ajpendo.00104.2003.

Link to article.

This is a GroPep specification. ibt is authorized distributor of GroPep.

Rev. 2013-02

 Datasheet for Chicken IGF-I, recombinant protein (Receptor Grade)