Product Specifications Biotinylated Human IGFBP-3:

The product: Biotinylated human IGFBP-3 has been prepared from receptor grade recombinant human IGFBP-3 (product code BP3BU020) using the ibt biotinylation technology.

The product was purified using chromatographic techniques.

Formulation: Lyophilized with no additives.

Reconstitution: Reconstitute 2.5 ug aliquot with 0.1 ml double distilled water or buffer of your choice.

Storage: The product is shipped at room temperature, for long term storage store at – 20 °C. Reconstituted samples can be stored for at least one week at 2-8 °C.

References on the use of biotinylated IGFBP-3:

1.) Puvogel et al.: Effects of an Enhanced Vitamin A Intake During the Dry Period

on Retinoids, Lactoferrin, IGF System, Mammary Gland Epithelial Cell Apoptosis, and Subsequent Lactation in Dairy Cows. J. Dairy Sci. 88:1785–1800. American Dairy Science Association, 2005.

Link to article:

http://jds.fass.org/cgi/reprint/88/5/1785

2.) Leal et al.: Interactions of High Affinity Insulin-like Growth Factor-binding

Proteins with the Type V Transforming Growth Factor-b Receptor in Mink Lung Epithelial Cells. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 274, No. 10, Issue of March 5, pp. 6711–6717, 1999.

Link to article:

http://www.jbc.org/cgi/reprint/274/10/6711

3.) Suresh, M. and Murphy L.M.: Phosphorylation of Insulin-Like Growth Factor (IGF) Binding Protein-3 by Breast Cancer Cell Membranes Enhances IGF-I Binding. Endocrinology Vol. 144, No. 9 4042-4050.

Link to article:

http://endo.endojournals.org/cgi/content/full/144/9/4042#F2

4.) Suresh, M. and Murphy L.M.: The effects of insulin-like growth factor binding protein-3 (IGFBP-3) on T47D breast cancer cells enriched for IGFBP-3 binding sites. Mol Cell Biochem. 2004 Dec;267(1-2):83-9.

Link to abstract:

http://www.ncbi.nlm.nih.gov/pubmed/15663189

5.) Gui, Y and Murphy, L. M.: Insulin-Like Growth Factor (IGF)-Binding Protein-3 (IGFBP-3) Binds to Fibronectin (FN): Demonstration of IGF-I/IGFBP-3/FN Ternary Complexes in Human Plasma. The Journal of Clinical Endocrinology & Metabolism Vol. 86, No. 5 2104-2110.
Link to article:

http://jcem.endojournals.org/cgi/content/full/86/5/2104

6.) Brand-Miller et al.: The glycemic index of foods influences postprandial insulin-like growth factor–binding protein responses in lean young subjects.

American Journal of Clinical Nutrition, Vol. 82, No. 2, 350-354, August 2005
Link to article:

http://www.ajcn.org/cgi/content/full/82/2/350

7.) Mishra et al.: Insulin-Like Growth Factor Binding Protein-3 Interacts with Autocrine Motility Factor/Phosphoglucose Isomerase (AMF/PGI) and Inhibits the AMF/PGI Function. CANCER RESEARCH 64, 2516–2522, April 1, 2004

Link to article:

http://cancerres.aacrjournals.org/cgi/reprint/64/7/2516.pdf

8.) Suresh, M. and Murphy L.M.: IDENTIFICATION OF TRANSGLUTAMINASE 2 AS AN INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-3 KINASE. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 279, No. 23, Issue of June 4, pp. 23863–23868, 2004

Link to article:

http://www.jbc.org/cgi/reprint/279/23/23863

9.) Baumrucker et al.: The Insulin-like Growth Factor (IGF) System in Mammary Gland: Role of IGFBP-3 Binding Protein. In: Ruminant Physiology: Digestion, Metabolism, Growth, and Reproduction. By Pierre Cronjé, E. A. Boomker. Contributor Pierre Cronjé. Published by CABI Publishing, 2000, ISBN 0851994636, 9780851994635

A preview of the book is available at:

http://books.google.com/books?id=_cxUuEaITRsC&pg=PA343&lpg=PA343&dq=biotinylated+IGF-&source=web&ots=DW09t7fwLM&sig=W4uDm1hxfPevSdjixJwCkHzJmoc&hl=en&sa=X&oi=book_result&resnum=5&ct=result

10.) Campbell et al: Insulin-like Growth Factor-binding Protein-3 Binds Fibrinogen and Fibrin. The Journal of Biological Chemistry October 15, 1999, 274, 30215-30221.

Link to article: www.jbc.org/content/274/42/30215.full

See also Application Note IGF015: Applications of Biotinylated (Biotin-labeled) IGFBP´s.

Rev2013-03

 Datasheet for Human IGFBP-3, recombinant protein, Biotin-labeled (Biotinylated IGFBP-3)