ibt – immunological and biochemical testsystems GmbH: innovative tools for functional proteomics are our target!
Product Search Results
Rat IGF-I, recombinant protein (Receptor Grade)
Insulin-like Growth Factor-I, Rat, Receptor Grade, recombinant Synonyms: Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor
Product Specifications:
The Product: Rat IGF-I is a polypeptide comprising of 70 amino
acids, three of which differ from the amino acid sequence of
human, IGF-I. Rat IGF-I has been shown to stimulate growth in rat
L6 myoblasts and other cell lines.
Source: Rat IGF-I is produced recombinantly in E.coli using a
patented expression system. The rat IGF-I molecule is correctly
folded and several chromatography purification steps are then
employed to yield Receptor Grade rat IGF-I.
Purity: > 95% (as determined by HPLC and N-terminal
sequence analysis)
Molecular Weight: 7687 daltons - confirmed by Mass
Spectrometry
N-terminal sequence analysis of recombinant product: 5
residues >95% single sequence
Biological Activity:(stimulation of protein synthesis in rat L6
myoblasts) ED50 <30 ng/ml
Endotoxin (LAL): <0.1 EU/ug
Stability: at least 2 years at 2-4°C (lyophilised)
Appearance: White Powder
State: Dried from 0.1 M acetic acid: under dry nitrogen @ slight
vacuum (-25kPa)
Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M
acetic acid), aliquot and store at -20°C. For lower dilutions (e.g.
<1mg/ml) inclusion of a carrier protein is recommended. Avoid
freeze-thaw cycles.
Reference:
1.) Ciucci et al.: Insulin-Like Growth Factor 1 (IGF-1) Mediates the Effects of Enriched Environment (EE) on Visual Cortical Development.
PLoS ONE. 2007; 2(5): e475. Published online 2007 May 30. doi: 10.1371/journal.pone.0000475.
2.) Nishijima et al.: Neuronal Activity Drives Localized Blood-Brain-Barrier Transport of Serum Insulin-like Growth Factor-I into the CNS. Neuron 67, 834–846, September 9, 2010.
Link to Article:
http://digital.csic.es/bitstream/10261/27623/1/NeuronCSICRepository.pdf
This is a GroPep specification. ibt is authorized distributor of GroPep.
Rev. 2013-02
Datasheet for Rat IGF-I, recombinant protein (Receptor Grade)