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Mouse IGF-I, recombinant protein
Recombinant Murine Insulin Like Growth Factor-I Synonyms: Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor
Product Specifications Mouse IGF-I:
Recombinant Murine Insulin Like Growth Factor-I (IGF-1)
Source: E.Coli
Product Code: rmIGF-I
Background: Murine IGF-I is a 70 amino acid peptide. Four of them differ from the human sequence, one is different from rat IGF-I.
Description: Recombinant murine IGF-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton. IGF-I is purified by chromatographic techniques.
Formulation: Sterile Filtered White lyophilized (freeze-dried) powder. Recombinant Murine IGF-1 was lyophilized after extensive dialysis against PBS.
Solubility: It is recommended to reconstitute the lyophilized Murine IGF-I in sterile double distilled water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability: Lyophilized Murine IGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18 °C. Upon reconstitution Murine IGF-1 should be stored at 4 C between 2-7 days and for future use below -18 °C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino acid sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically.
Dimers and aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
Biological Activity: Murine IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is less then 1.0 ng/ml, corresponding to a specific activity of 1x10 U/mg.
Endotoxin: Less than 0.1 ng/µg (IEU/µg) of IGF-I.
Reference:
1. Fernandez et al.: Calcineurin in Reactive Astrocytes Plays a Key Role in the Interplay between Proinflammatory and Anti-Inflammatory Signals. The Journal of Neuroscience, August 15, 2007, 27(33):8745-8756.
Datasheet for Mouse IGF-I, recombinant protein