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LR3 IGF-I, recombinant protein (Receptor Grade)
LR3- IGF-I,
Product Specifications LR3IGF-I (Receptor Grade):
The Product: LR3 insulin-like growth factor-I is an 83 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Arg (R) for the Glu (E) at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. GroPeo sientists have engineered this analog with the express purpose of increasing iological activity. LR3IGF-I is significantly more potent than IGF-I in vitro and in vivo. The enhanced potency is due to the markedly decreased binding of LR3 IGF-I to all IGF binding proteins. These binding proteins normally inhibit the biological actions of IGFs.
Source: LR3 IGF-I is produced in E. coli using a patented expression system the LR3 IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade LR3IGF-I.
Purity: >95% (as determined by HPLC and N-terminal sequence analysis)
Molecular Weight: 9111.6 daltons - confirmed by Mass Spectrometry
N-terminal sequence analysis of recombinant product: 18 residues >95% single sequence
Biological Activity: (stimulation of protein synthesis in rat L6 myoblasts) ED50 <10 ng/ml
Endotoxin (LAL): <0.1 EU/ug
Storage/Stability: At least 2 years at 2-4°C(lyophilised)
Appearance: White Powder
State: Dried from 0.1 M acetic acid: under dry nitrogen at slight vacuum (-25kPa)
Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended. Avoid repeated freeze-thaw cycles.
Rev. 2013-02
Datasheet for LR3 IGF-I, recombinant protein (Receptor Grade)