Product Specifications [Leu60]IGF-I:  

[Leu60]IGF-I is an analog of human IGF-I with a substitution of Leu for Tyr
at position 60 of the human IGF-I sequence. Compared to IGF-I, [Leu60]IGF-I has greatly reduced binding to both the Type 1 IGF receptor and the insulin receptor, but binding to acid-stripped human serum    binding proteins is unaltered. Consequently, [Leu60]IGF-I is an extremely    useful analog of IGF-I for investigating IGF actions largely independent of    binding to the Type 1 IGF receptor. [Leu60]IGF-I has a lower affinity for    the Type 1 IGF and the insulin receptors than  [Leu24]IGF-I.    

Source:[Leu60]IGF-I is produced recombinantly as a fusion protein in E.coli using a patented expression system. The fusion protein containing  [Leu60]IGF-I is refolded, and after the leader peptide is removed, the [Leu60]IGF-I product is purified to Receptor Grade using several chromatography steps.    

Purity:> 95% (as determined by HPLC and N-terminal sequence analysis) Molecular weight: Theoretical : 7599 daltons. Confirmed by Mass Spectrometry.

Appearance:Lyophilized white powder 

N-terminal sequence analysis: 5 residues > 95% single sequence   Endotoxin (LAL): < 0.1 EU/µg    

Biological Activity:

Type 1 IGF receptor binding assay - ED50 > 150 ng/ml                         IGF binding protein assay - ED50 < 10 ng/ml                       
Stimulation of protein synthesis in rat L6 myoblasts - ED50 > 2000 ng/ml 

State:Dried from 0.1 M acetic acid and stored under dry nitrogen at a slight vacuum (-25kPa).    

Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. < 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.

Storage/Stability: At least 2 years at 2-4°C (lyophilized)

Peptide can be biotinylated by ibt upon request.

This is a GroPep specification. ibt is authorized distributor of GroPep.

Rev. 2013-02

 Datasheet for (Leu60)IGF-II, recombinant protein (Receptor Grade)