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(Leu27) IGF-II, recombinant protein (Receptor Grade)
(Leu27) Insulin-like Growth Factor-II, recombinant (Receptor Grade) Synonyms:(Leu27) Insulin-like Growth Factor-2, (Leu27) IGF-2
Product Specifications (Leu27) IGF-II:
[Leu27]IGF-II is an analog of human IGF-II which has a Leu(L) substitution in place of Try(Y) at position 27 of the IGF-II amino acid sequence. Compared to IGF-II, [Leu27]IGF-II has a 1000- fold reduced affinity for the type 1 receptor.
Source: [Leu27]IGF-II is produced recombinantly in E.coli using a patented expression system, and the [Leu27]IGF-II is refolded under oxidising conditions and a short leader peptide is cleaved. Several chromatography purification steps are employed to yield Receptor Grade [Leu27]IGF-II.
Purity: >95% (as determined by HPLC and N-terminal sequence analysis)
Molecular Weight: 7420 daltons, confirmed by mass spectroscopy
N-terminal sequence analysis of recombinant product:Sequence checked to 27 residues and <95% single sequence.
Biological Activity: [Leu27]IGF-II in a type 2 IGF receptor binding assay using rat L6 myoblasts showed an ED50 of 16 nM, compared to 8 nM for IGF-II. Compared to IGF-II,[Leu27]IGF-II has a 1000 fold reduced affinity for the type 1 IGFreceptor.
Endotoxin (LAL): <0.01 EU/mg
Stability: At least 2 years at 2-4°C (lyophilised)
Appearance: Lyophilized white powderState: Dried from 0.1M acetic acid:under dry nitrogen at slight vacuum (-25kPa)Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g.<1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles. Peptide can be biotinylated by ibt upon request. CAUTION: Not for human or in-vitro diagnostic use! This is a GroPep specification. ibt is authorized distributor of GroPep.
Rev. 2013-02
Datasheet for (Leu27) IGF-II, recombinant protein (Receptor Grade)