Product Specifications Biotin-Pro-IGF-II:

Biotin-Pro-IGF-II is prepared by a selective biotinylation procedure. Affinity of the biotinylated precursor was compared with biotinylated IGF-II. Scientific papers on affinity of the precursor to IGFBP´s are somewhat controversial. We found no significant difference of affinities of biotinylated IGF-II or biotinylated pro-IGF-II to IGFBP-3 in western-ligand blotting experiments. This is in agreement with the results of Zapf (J. Zapf, IGFs: function and clinical importance, 3 Role of insulin-like growth factor (IGF) II and IGF binding proteins in extrapancreatic tumor hypoglycemia. J Intern Med 1993;234; 543-552).

Formulation: Lyophilised from a solution of  0.5 ml TBS, pH 7.4, containing
4 µg of the biotinylated product . A  preservative and a stabilising protein of non-mammalian origin are added to ensure stability of the reconstituted product.

Recommended dilution: 1: 100 to 1: 500 for western ligand blotting. Tested with our western-ligand blotting kit.

Storage: Store refrigerated upon arrival. The product is stable at 4 °C for
at least one year.  Reconstitute with 0.5 ml water and store aliquots frozen at - 20 °C. Avoid repeated freeze-thawing cycles. 

Rev. 2013-02

 Datasheet for Human Pro-IGF-II (1-156), Biotinylated (Biotin-Pro-IGF-II)