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Human IGFBP-5, recombinant protein (Receptor Grade)
Insulin-like Growth Factor Binding Protein-5, human, recombinant Synonyms: IGF binding protein-5, Ibp5
Product specifications Human IGFBP-5:
Human Insulin-like Growth Factor Binding Protein-5 (rhIGFBP-5) is a 252 amino acid glycosylated protein that is one of the six 'classical' IGF binding proteins. These binding proteins have an important role in stabilizing and regulating the activity of IGFs in vivo. Human Insulin-Like Growth Factor Binding Protein-5 (hIGFBP-5) is a 252 amino acid protein that is one of the six 'classical' IGF binding proteins that play an important role in stabilizing and regulating the activity of IGFs in vivo. Like IGFBP-3 it forms a ternary complex with an acid-labile subunit (ALS). hIGFBP-5 preferentially binds IGF-II and it also binds with high affinity to extracellular matrix components which protect it from proteolysis. IGFBP-5 has a nuclear targeting sequence and, independent of the presence of IGF-I, fluorescently labelled IGFBP-5 is translocated to the nucleus of actively dividing cells. The significance of this observation is not yet understood.
References:
Kiefer, M. et al. (1991) Biochem. Biophys. Res. Commun. 176, 219-225
Twigg, S.M. et al. (1998) J. Biol. Chem. 273, 28791-28798
Source: Produced recombinantly in mammalian cells. Purified by affinity and other chromatographic steps.
Purity: > 95% human IGFBP-5 (as determined by HPLC and N-terminal sequence)
Molecular weight:31-32 kDa
Quantity: 20 µg
Appearance:Lyophilized white powder
Biological Activity:Detected by immunoblotting using an antibody specific for IGFBP-5.Binds biotinylated IGF-I and -II.
State: Dried from 1M acetic acid
Reconstitution: Reconstitute in 50 µl 10 mM HCl at a concentration of 0.2 mg/ml. Aliquot, snap freeze and store at -80°C. Avoid freeze-thaw cycles.
Storage/Stability: At least 2 years at 2-4°C (lyophilized)
Ref:
Hsieh et al.:Regulation of Vascular Smooth Muscle Cell Responses to Insulin-like Growth Factor (IGF)-I by Local IGF-binding Proteins.J. Biol. Chem., Vol. 278, Issue 44, 42886-42892, October 31, 2003
Link to article: http://www.jbc.org/cgi/content/full/278/44/42886.
This a GroPep Specification! ibt is authorized distributor of GroPep!
Rev2013-03
Datasheet for Human IGFBP-5, recombinant protein (Receptor Grade)