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Human IGF-II (Receptor Grade), recombinant protein
Insulin-like Growth Factor-II, human, Synonyms: insulin like growth factor-2, IGF-2, Somatomedin A, ILGF2, T3M-11-derived growth factor,
Product Specifications Human IGF-II:
The Product: Human insulin-like growth factor-II (IGF-II) is a 67amino acid polypeptide that stimulates growth and differentiationin many cell types.
Source: IGF-II is synthesized in E. coli using a patented expression system and harvested from the cells as inclusion bodies. The inclusion bodies are dissolved, desalted, the IGF-II is correctly folded under oxidising conditions and then a short leader peptide is cleaved from the peptide product. Several liquid chromatography purification steps are employed to yield receptor grade IGF-II.
Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)
Molecular Weight: 7469 daltons - confirmed by Mass Spectrometry
N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence
Biological Activity: (stimulation of protein synthesis in L6 myoblasts) ED50 < 150 ng/ml
Endotoxin (LAL): <0.1 EU/ug
Stability: At least 2 years at 2-4°C(lyophilised)
Appearance: White Powder
State: Dried from 0.1 M acetic acid: under dry N2 at slightvacuum (-25kPa)
Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -800C. For lower dilutions (e.g.<1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.
This is a Gropep specification. ibt is authorized distributor of GroPep.
Rev. 2013-02
Datasheet for Human IGF-II (Receptor Grade), recombinant protein