Product Specifications Human IGF-II:

The Product: Human insulin-like growth factor-II  (IGF-II) is a 67amino acid polypeptide that stimulates growth and differentiationin many cell types.

Source: IGF-II is synthesized in E. coli using a patented expression system and harvested from the cells as inclusion bodies.  The inclusion bodies are dissolved, desalted, the IGF-II is correctly folded under oxidising conditions and then a short leader peptide is cleaved from the peptide product.  Several liquid chromatography purification steps are employed to yield receptor grade IGF-II.

Link to Sequence:

Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)

Molecular Weight: 7469 daltons - confirmed by Mass Spectrometry

N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence

Biological Activity: (stimulation of protein synthesis in L6 myoblasts) ED50  < 150 ng/ml

Endotoxin (LAL): <0.1 EU/ug

Stability: At least 2 years at 2-4°C(lyophilised)

Appearance: White Powder

State: Dried from 0.1 M acetic acid: under dry N2 at slightvacuum (-25kPa)

Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -800C.  For lower dilutions (e.g.<1mg/ml) inclusion of a carrier protein is recommended.  Avoid freeze-thaw cycles.

This is a Gropep specification. ibt is authorized distributor of GroPep.

Rev. 2013-02

 Datasheet for Human IGF-II (Receptor Grade), recombinant protein