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Human IGF-II, Biotinylated (Biotin- IGF-II)
Insulin-like Growth Factor-II, human, Biotinylated Synonyms: Insulin-like Growth Factor-2, human, Biotinylated; Biotin-IGF-2
Product Specifications Biotin- IGF-II:
Biotin- IGF-II is prepared by a selective biotinylation procedure,
that ensures full binding capacity to all six IGF-binding proteins. Binding
to antiserum PAC is not affected.
The use of biotinylated IGF-II is described in application note 5
Examples of applications of biotinylated IGF's can be seen in application note 4.
Formulation: Lyophilised from a solution of 0.5 ml TBS, pH 7.4, containing
100 ug/ml of the biotinylated product . A preservative and a
stabilising protein of non-mammalian origin are added to ensure stability
of the reconstituted product.
Recommended dilution: 1: 100 to 1: 500 for western ligand blotting. Tested with our western-ligand blotting kit.
Storage: Store refrigerated upon arrival. The product is stable at 4 °C for at least one year. Reconstitute with 0.5 ml water and store aliquots frozen at - 20 °C. Avoid repeated freeze-thawing cycles.
Reference:
Suresh Koduru, Sivaramakrishna Yadavalli, Siva Kumar Nadimpalli:
Mannose 6-phosphate Receptor (MPR 300) Proteins
from Goat and Chicken Bind Human IGF-II
Published online:Biosci Rep (2006) 26:101–112.
Link to article: http://www.bioscirep.org/bsr/026/0101/0260101.pdf.
Rev. 2013-02
Datasheet for Human IGF-II, Biotinylated (Biotin- IGF-II)