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Human IGF-I, recombinant protein (Media Grade)
Insulin-like Growth Factor-I, recombinant, human (Receptor Grade) Synonyms: Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor
Product Specifications Human IGF-I:
Insulin-like growth factor-I (IGF-I) is a 70 amino acid polypeptide that stimulates growth and differentiation in many cell types including rat L6 myoblasts.
Source: IGF-I is produced in E. coli using a patented expression system and the IGF-I is correctly folded and purified by several chromatography steps to yield Receptor Grade IGF-I.
Purity: > 85% (as determined by HPLC and N-terminal sequence analysis)
Molecular Weight: 7649 daltons - confirmed by Mass Spectrometry
N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence
Biological Activity: (stimulation of protein synthesis in rat L6 myoblasts) ED50 <30 ng/ml
Endotoxin (LAL): <0.1 EU/ugStorage/Stability: At least 2 years at 2-4°C (lyophilised)
Appearance: White Powder
State: Dried from 0.1 M acetic acid: under dry nitrogen at slight vacuum (-25kPa)
Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. <1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.
This is a GroPep specification. ibt is authorized distributor of GroPep Ltd.
Reference:
1. Granata et al. (2007): Insulin-like growth factor binding protein-3 induces angiogenesis through IGF-I- and SphK1-dependent mechanisms. Journal of Thrombosis and Haemostasis. Volume 5, Issue 4, Pages 835-845.
2. Ciucci F, Putignano E, Baroncelli L, Landi S, Berardi N, et al. (2007) Insulin-Like Growth Factor 1 (IGF-1) Mediates the Effects of Enriched Environment (EE) on Visual Cortical Development. PLoS ONE 2(5): e475 doi:10.1371/journal.pone.0000475
Rev. 2013-02
Datasheet for Human IGF-I, recombinant protein (Media Grade)