Product Specifications Biotin-Des(1-6) IGF-II:

Biotin-Des(1-6) IGF-II is prepared by a selective biotinylation procedure. As the unbiotinylated analog it has onyl very weak affinity to  IGF-binding proteins. Binding to recpetors is not substantially affected. Due to its strongly reduced binding affinity to the IGFBP´s, Biotin - Des (1-6) IGF-II may be used for detection of IGF-II receptors on western blots, in binding studies or in immunohistochemistry.

Examples of applications of biotinylated IGF's can be seen in application note 4.

Formulation: Lyophilised from a solution of  0.5 ml TBS, pH 7.4, containing
10 µg of the biotinylated product . A  preservative and a
stabilising protein of non-mammalian origin are added to ensure stability
of the reconstituted product.
Recommended dilution: 1: 100  for western ligand blotting. Tested with
our western-ligand blotting kit.
Storage: Store refrigerated upon arrival. The product is stable at 4 °C for
at least one year.  Reconstitute with 0.5 ml water and store aliquots frozen at - 20 °C. Avoid repeated freeze-thawing cycles.

Rev. 2013-02

 Datasheet for Des(1-6) IGF-II, Biotinylated (Biotin-Des(1-6) IGF-II)