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Chicken IGF-II, recombinant protein (Receptor Grade)
Insulin-like Growth Factor-II, Chicken, Receptor Grade, recombinant Synonyms: insulin like growth factor-2, IGF-2, Somatomedin A, ILGF2, T3M-11-derived growth factor,
Product Specifications Chicken IGF-II:
The Product: Chicken IGF-II is a polypeptide comprising of 66 amino acids, twelve of which differ from the amino acid sequence of human IGF-II. Chicken IGF-II has been shown to stimulate growth and differentiation in most cell types, including L6 myoblasts and chicken embryo fibroblasts.
Source: Chicken IGF-II is produced in E. coli and several purification steps are employed to yield Receptor Grade chicken IGF-II.
Purity: >95% (as determined by HPLC)
Molecular Weight: 7512 daltons - confirmed by Mass Spectrometry
N-terminal sequence analysis of recombinant product: 5 residues >95% single sequence.
Biological Activity: (Stimulation of protein synthesis in L6 myoblasts) ED50 <150 ng/ml.
Endotoxin (LAL): <0.1 EU/ug
Storage/Stability: At least 2 years at 2-4° C (lyophilised)
Appearance: White Powder
State: Dried from 0.1 M acetic acid: under dry nitrogen @ slight vacuum (-25kPa)
Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g.<1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.
Reference: Effects of Genotype and Feed Allowance on Plasma Luteinizing Hormones, Follicle-Stimulating Hormones, Progesterone, Estradiol Levels, Follicle Differentiation, and Egg Production Rates of Broiler Breeder Hens. Ongabesan et al. Link to Article.
Peptide can be biotinylated by ibt upon request.
This is a Gropep specification. ibt is authorized distributor of GroPep.
Datasheet for Chicken IGF-II, recombinant protein (Receptor Grade)