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Bream IGF-I, recombinant protein (Receptor Grade)
Bream (Pagrus auratus) Insulin-like Growth Factor-I (Receptor Grade) Synonyms: Igf-1, insulin-like growth factor-1, Somatomedin c, ILGF1, Mechano growth factor
Product Specifications Bream IGF-I:
Recombinant red sea bream (Pagrus auratus) insulin-like growth factor-I s a 67 amino acid polypeptide. IGF-I stimulates growth and differentiation in many cell types and mediates many of the anabolic properties of growth hormone. Recombinant red sea bream (Pagrus auratus) IGF-I is identical to [Gly1] gilthead sea bream (Sparus aurata) IGF-I and [Gly1] black bream (Acanthopagrus butcheri) IGF-I, as glycine replaces the serine at position 1 in these proteins.
Source: Red sea bream IGF-I is synthesized in E. coli using a patented expression system and the bream IGF-I is correctly folded and then purified by several liquid chromatography purification steps to yield receptor grade bream IGF-I.
Purity: > 95% (as determined by HPLC and N-terminal sequence analysis)
Molecular Weight:
7379 daltons – confirmed by Mass Spectrometry
Appearance: White Powder
N-terminal sequence analysis: 5 residues > 95% single sequence
Endotoxin (LAL): < 0.1 EU/µg
Biological Activity: (stimulation of protein synthesis in L6 myoblasts) ED50 < 5 nM
State: Lyophilised from 0.1 M acetic acid: under dry nitrogen at a slight vacuum (-25kPa)
Reconstitution: Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C. For lower dilutions (e.g. < 1mg/ml) inclusion of a carrier protein is recommended. Avoid freeze-thaw cycles.
Storage/Stability: At least 2 years at 2-4°C (lyophilised)
This is a Gropep specification. ibt is authorized distributor of GroPep.
Rev. 2013-02
Datasheet for Bream IGF-I, recombinant protein (Receptor Grade)