Product Specifications Bovine IGFBP-2:

Recombinant bovine IGF Binding Protein-2 (bIGFBP-2) is a 284 amino acid protein that is a member of a family of six high affinity IGF binding proteins. These binding proteins have an important role in stabilizing and regulating the activity of IGFs in vivo. Bovine IGFBP-2 is able to bind both IGF-I and IGF-II.

Link to Sequence.
References:

Upton, Z. et al (1990) J. Mol. Endo. 5, 77-84.
Clemmons, D.R. et al (1992) Endocrinology 131, 890-895.
Bourner, M.J. et al (1992) J. Cell. Biochem. 48, 215-226.

Source: Produced recombinantly in mammalian cells. Purified by affinity and other chromatographic steps.

Purity: > 95% (as determined by HPLC, N-terminal sequence and Mass Spectrometry).

Molecular weight: 30776 daltons. Confirmed by Mass Spectrometry.

Appearance: Lyophilized white powder

N-terminal sequence analysis: 5 residues > 95% single sequence

Biological Activity: IGF-I Binding Assay: ED50 < 10 nM

State: Dried from 0.1% TFA / 30% acetonitrile

Reconstitution: Reconstitute in 100 µl of 10 mM HCl at a concentration of 0.2 mg/ml. Aliquot, snap-freeze and store at -80°C. Avoid freeze/thaw cycles.

Storage/Stability: At least 2 years at 2-4°C (lyophilised)
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Rev2013-03

 Datasheet for Bovine IGFBP-2, recombinant protein(receptor grade)