Product Specifications Barramundi IGF-I:

The Product:  Recombinant barramundi (Lates calcarifer) insulin-like growth factor-I  (rbIGF-I) is a 66 amino acid polypeptide.  IGF-I stimulates growth and differentiation in many cell types.  IGF-I mediates many of the anabolic properties of growth hormone.

Source:  Barramundi  IGF-I is synthesized in E. coli using a patented expression system and the barramundi IGF-I is correctly folded and then purified by several liquid chromatography purification steps to yield receptor grade barramundi IGF-I.

Purity:  > 95% (as determined by HPLC and N-terminal sequence analysis).

Molecular Weight: 7309 daltons – confirmed by Mass Spectrometer.

N-terminal sequence analysis of recombinant product:  5 residues  >  95% single sequence.

Biological Activity: (stimulation of protein synthesis in L6 myoblasts) ED50:24.0 ng/ml

Endotoxin (LAL):   < 0.1 EU/µg

Stability:  At least 2 years at 2-4°C (lyophilised)

Appearance:  White Powder

State:  Lyophilised from 0.1 M acetic acid: under dry nitrogen @ slight  vacuum (-25kPa).

Reconstitution:  Dissolve in dilute acid (e.g. 10mM HCl or 0.1M acetic acid), aliquot and store at -20°C.  For lower dilutions (e.g.< 1mg/ml) inclusion of a carrier protein is recommended.  Avoid freeze-thaw cycles.

This is a Gropep specification. ibt is authorized distributor of  GroPep.

Rev. 2013-02

 Datasheet for Barramundi IGF-I, recombinant protein (Receptor Grade)