ibt – immunological and biochemical testsystems GmbH: innovative tools for functional proteomics are our target!
Product Search Results
Anti-rat Insulin C-peptide, monoclonal, mouse
Several Clones available.
Product Specifications Monoclonal Mouse Anti-Rat Insulin C-Peptide:
Clones: 6H1, CII-11, CII-29, CII-55, CII-97, CII-106, CII-138, CI-0, CC24, CC27, CC34.
Description: Clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with fragments of rat C-peptides I and II conjugated with the carrier protein.
Specificity:
Clone | rat c-peptide I | rat c-peptide II | mouse c-peptide I | mouse c-peptide II | rat proinsulin |
CII-11, CII-29 | +++ | +++ | +++ | +++ | + |
CII-55, CII-97 | +++ | +++ | +++ | +++ | ++ |
CH1, CI-0 | ++ | low | low | ++ |
|
CII-106, CII-138 | - | +++ | low | +++ | not tested |
CC24, CC27, CC34 | +++ | +++ | +++ | + | - |
Isotype: IgG1 for Clones CII-11, CII-29, CII-55, CII-97, CII-106, CII-138, 6H1, CI-0, CC18, CC24, CC27, CC34.
Applications: All Clones recognize rat C-peptide in ELISA and Sandwich type immunoassay.
Clones can be used for separate or consistent detection of rat C-peptide isoforms (rat C-peptide I and/or rat C-peptide II) in biological samples.
Best pairs for sandwich immunoassays. For sandwich immunoassay the general recommendation is to use combinations of MAbs named as “CC†(MAbs specific to C-terminal part of rat C-peptide) and MAbs named as â€CI†or “CII†(MAbs specific to N-terminal part of rat C-peptide).
The most sensitive pairs recommended for sandwich-type rat C-peptide immunoassays are (capture-detection):
Rat C-peptides I and II immunodetection:
CII-29 – CC24, CII-55 – CC24, CII-97 – CC24, C34 – CII-11, CC27 – CII-29.
Rat C-peptide I immunodetection:
CC34 – 6H1, CC34 – CI-0, 6H1 – CC24.
Rat C-peptide II immunodetection:
CC18 – CII-106, CC34 – CII-106, CC34 – CII-138.
Purification: Chromatography on protein A Sepharose
Formulation: PBS, pH 7.4, < 0.1 % sodium azide (NaN3)
Storage:+ 4 °C.
Datasheet for Anti-rat Insulin C-peptide, monoclonal, mouse