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Anti-Progesterone, monoclonal, mouse
Several clones available.
Product Specifications Monoclonal Mouse Anti-Progesterone:
Clones: XM207, HPRO-1, HPRO-2, HPRO-3.
Description: Clone XM207 has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with conjugate of purified progesterone with BSA. For HPRO-1, HPRO-2 and HPRO-3 immunogen was conjugate of 17α-hydroxyprogesterone with BSA.
Specificity:
Clone XM207 - Progesterone, Ka = 3.0 x 109 M-1
Clone HPRO-2 - Progesterone
Clones HPRO-1, HPRO-3 - 17α-hydroxyprogesterone
Clone XM207 reacts with progesterone in ELISA. Clones HPRO-1, HPRO-2 and HPRO-3 react with 17α-hydroxyprogesterone-BSA conjugate and free 17α-hydroxyprogesterone. There is no cross-reactivity with BSA.
Cross reactivty % | XM207 | HPRO-1 | HPRO-2 | HPRO-3 |
Progesterone | 100 | 7 | 100 | 11 |
17-Hydroxyprogesterone | 1.0 | 100 | 10 | 100 |
11-Hydroxyprogesterone | 25 | N/A | N/A | N/A |
5-alpha-pregnane-3,20 dionene | 10.5 | N/A | N/A | N/A |
Corticosterone | 0.01 | N/A | N/A | N/A |
Pregnenolone | 0.9 | N/A | N/A | N/A |
Deoxycorticosterone | 0.30 | 3 | 1 | 15 |
Deoxycortisol | 0.03 | N/A | N/A | N/A |
Cortisol | 0.002 | N/A N | /A | N/A |
Isotypes: IgG2b for Clones XM207, HPRO-2 and HPRO-3, IgG3 for Clone HPRO-1
Applications: XM207 and HPRO-2 can be used for detection of progesterone.
HPRO-1 and HPRO-3 can be used in competitive assay of 17α-hydroxyprogesterone.
Purification: HPRO-1, HPRO-2 and HPRO-3 by chromatography on protein G Sepharose, and XM207 by ion exchange chromatography.
Formulation: HPRO-1, HPRO-2 and HPRO-3 in PBS, pH 7.4, < 0.1 % sodium azide (NaN3), XM207 in 0.15 M NaCl, 10 mM Tris, pH 7.5, 0.05% of sodium azide (NaN3).
Storage: + 4 °C.
Datasheet for Anti-Progesterone, monoclonal, mouse