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Anti-human/mouse/rat IGFBP-6, polyclonal antibody, rabbit
Anti-human/mouse/rat Insulin-like Growth Factor Binding Protein-6, polyclonal, rabbit
Product Specifcations Anti-human/mouse/rat IGFBP-6:
The Product: The IGFBP-6 IgG fraction was purified from a polyclonal antiserum obtained from rabbits immunized with a synthetic peptide of unique sequence from the central domain of human IGFBP-6. The antibody has been characterized for immunohistochemistry and EIA.
Quantity: 20 ug.
Specificity: In EIA the antibody does not cross-react with human IGFBPs 1-5.
Application: Characterized for the immunohistochemical detection of IGFBP-6 in paraffin embedded sections of embryonic rat tissues.
Titre: A final dilution of 1/500 for IHC with GroPep’s protocol using a biotinylated IgG secondary antibody and detection with ExtrAvidin conjugated Cy3. Use at a final dilution of ~1/3,000 for EIA.
Reconstitution: Dissolve in 400 µl sterile water (1/20 dilution). Dilute further in a buffer solution appropriate for the desired application.
Storage/Stability: In lyophilized form the antibody is stable for at least 2 years at 2 - 4 °C. After reconstitution, aliquot and store at -20 °C or -80°C. Avoid repeated freezing and thawing.
Reference: Lipinski et. al.:Sonic hedgehog signaling regulates the expression of insulin-like growth factor binding protein-6 during fetal prostate development. Developmental Dynamics 2005,Volume 233, Issue 3, Pages 829-836.
This is a Novozymes GroPep specification. ibt is authorized distributor of Novozymes GroPep!
Datasheet for Anti-human/mouse/rat IGFBP-6, polyclonal antibody, rabbit