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Anti-Human Insulin, monoclonal, mouse
Product Specifications Monoclonal mouse anti-human insulin:
Clones: C7C9, D4B8, 7F8, 3A6, 8E2, 7F5.
Description: Clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.
Specificity: Insulin, human. Clone C7C9 reacts with C-terminal pentapeptide of insulin beta-chain. Clones 7F8, D4B8, 3A6 and 8E2 react with different antigenic determinants.
These antibodies cross-react with human proinsulin, bovine insulin (30%) and porcine insulin. No cross-reaction with free C-peptide.
Isotype: IgG1, kappa for all Clones.
Applications: Detection of insulin in two-site enzyme immunoassay. Following pairs are recommended (coating - conjugate): 7F8 - D4B8, 3A6 - 8E2.
Clone D4B8 can be used in immunohistochemistry on frozen sections.
Purification: Chromatography on protein A Sepharose.
Formulation: PBS, pH 7.4, < 0.1 % sodium azide (NaN3).
Storage: + 4 °C.
Datasheet for Anti-Human Insulin, monoclonal, mouse