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Anti-Human IGFBP-1 Antiserum, Polyclonal (Rabbit)
Human IGFBP-1 antiserum is a polyclonal antiserum raised against a
synthetic peptide corresponding to amino acids 136-147 (Zapf, J. (1995)
Eur. J. Endocrinol. 132, 645-654) of human IGFBP-1. This antiserum is
suitable for use in ELISA and Western blotting and should find
application in immunohistochemistry.
Source:
The antiserum was obtained from rabbits immunized
against a synthetic peptide corresponding to amino
acids 136-147 (Zapf, J. 1995) of human IGFBP-1.
Quantity:
100 µl of serum
Appearance:
White powder
Specificity:
No crossreactivity is found with human IGFBP-2,
IGFBP-3 or IGFBP-4.
Titre:
Can be used at a dilution of 1/4,000 in ELISA.
Half-maximal inhibition (ED50) is produced by 28 ng
hIGFBP-1 / 100µl. For Western blotting use a dilution
of 1/1,000.
Reconstitution:
Dissolve in 100 µl sterile water. Dilute in a suitable
buffer solution appropriate for the desired application.
Storage/Stability:
At least 2 years at 2 -4°C (lyophilised). After
reconstitution, aliquot and store at -20°C. Avoid
repeated freezing and thawing.
Datasheet for Anti-Human IGFBP-1 Antiserum, Polyclonal (Rabbit)