Product Specifications Anti-human IGF-I:

Mouse monoclonal (Type IgG1/kappa)

Quantity: 500 µg

Immunogen:Recombinant human IGF-I (Insulin-like Growth Factor-I)

State and Appearance:Lyophilized powder

Specificity:As determined by EIA:
100% human Des(1-3)IGF-I, Long^TMR³IGF-I
<4% rat IGF-I
<0.4% human IGF-II, chicken IGF-I, salmon IGF-I

Storage/Stability:At least 2 years at 2 - 4°C (lyophilized). After reconstitution, aliquot and store at -20°C. Avoid freeze-thaw cycles

Reconstitution: Reconstitute the vial contents in 500 µl sterile water. Allow to stand at room temperature for at least 10 minutes before use. Dilute in a buffer solution suitable for the desired application.

Application and Titre:Characterized for EIA (1/160) and immunoblotting (1/100). Detects 60 ng human IGF-I in western immunoblot (at a final dilution of 1/100). After reconstitution, a dilution of 1/160 will give an absorbance of 1.0 at 490nm when reacted with 25 ng/well of human IGF-I, a 1/2,000 dilution of HRP-labelled anti-mouse IgG (DAKO PO447) & a 1 mg/ml OPD substrate solution.

This is a Novozymes GroPep specification. ibt is authorized distributor of Novozymes GroPep!

 Datasheet for Anti-human IGF-I, monoclonal antibody, mouse