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Product Specifications Bream (Acanthopagrus butcheri) Growth Hormone Polyclonal Antiserum (Rabbit):

The Product: Anti-bream (Acanthopagrus butcheri) growth hormone (GH) antiserum is a polyclonal antiserum raised against recombinant bream GH.  This antiserum has been used for radioimmunoassay and is suitable for immunohistochemistry and Western blotting.

Source:  The antiserum was obtained from a rabbit immunised with recombinant bream  growth hormone.

Quantity:  15 µl of serum

Appearance:  White powder

Specificity: The antiserum displays less than 0.2% cross-reactivity with recombinant  trout (Oncorhynchus mykiss) GH, recombinant goldfish (Carassius auratus) GH and recombinant human, rat and porcine GH.

Reconstitution:  Dissolve in a suitable buffer solution appropriate for the desired application.

Storage/Stability:  At least 2 years at 2-4°C (lyophilised).  After reconstitution, aliquot and store at -20°C.  Avoid repeated freezing and thawing.

 Applications: Radioimmunoassay. For radioimmunoassay (RIA), the antiserum is used at a final dilution of    1/20,000.  At this dilution the antiserum binds 20-30% of fresh [125I]    Bream GH tracer (80 Ci/g).  Half maximal inhibition (ED50) of radioligand binding is produced by 7.0 ng/ml of bream GH.
This a Novozymes GroPep Specification! ibt is authorized distributor of Novozymes GroPep!

 Datasheet for Anti-Growth Hormone, Bream (Pagrus auratus), polyclonal, rabbit