Product Specifications Anti-human IGF-I:

Anti-human IGF-I is a polyclonal antiserum specific for human, bovine and porcine IGF-I all of which share an identical amino acid sequence. This antiserum is suitable for use in radioimmunoassay, immunoblotting and immunoprecipitation.

Source: The antiserum is derived from rabbit serum raised against an immunogenic analog of human IGF-I.
Quantity: 5 µl of serum
Appearance: White Powder

Specificity: The antiserum is specific for human, bovine or porcine IGF-I. Cross- reactivity with rat IGF-I and chicken IGF-I is 25% and 70%  respectively. The antiserum displays less than 1 % cross-reactivity with human IGF-II.

Titre: At a dilution of 1/80 000 the antiserum binds 30-40% of fresh [125I]-labelled human IGF-I tracer.  Half-maximal inhibition (ED50) of radioligand binding is produced by 250-300 pg of human IGF-I.

Reconstitution: Dissolve in a suitable buffer  solution appropriate for desired application.

Storage/Stability: At least 2 years at 2-4°C (lyophilised).  After
reconstitution store at -20°C.

The relative potencies of the following peptides at inhibiting the
binding of [125I] human IGF-I to this antiserum were found to be:
human IGF-I:     100%
chicken IGF-I:    70%
bovine IGF-I*:   100%
Long [R3]IGF-I:  20%
porcine IGF-I*:  100%
[R3]IGF-I:         100 %
rat IGF-I:          25%
human IGF-II:    <1%
*NB: bovine and porcine IGF-I have the same amino acid sequence as human IGF-I.

 Datasheet for Anti-human IGF-I, polyclonal antiserum, rabbit