Product Specifications Anti-human/rat IGFBP-4:

Affinity purified for IHC: Working dilution: 1:250.
The Product:  The IgG fraction was purified from a polyclonal antiserum obtained from rabbits immunized with a synthetic peptide of unique sequence from the central domain of human IGFBP-4.  The antibody has been characterized for use in immunohistochemistry and EIA.
Quantity:  20 ug.
Specificity:  In EIA the antibody does not cross-react with human IGFBP-1, -2, -3, -5 and –6.
Application: Characterized for the immunohistochemical detection of IGFBP-4 in paraffin embedded sections of embryonic rat tissues.
Titre:  A final dilution of 1/250 for immunohistochemistry with GroPep’s protocol using a biotinylated IgG secondary antibody and detection with ExtrAvidin conjugated Cy3.
Use at a final dilution of 1/1,000 - 1/2,000 for EIA.
Reconstitution:  Dissolve in 400 µl sterile water (a 1/20 dilution). Dilute urther in a buffer solution appropriate for the desired application.
Storage/Stability: In lyophilized form, the antibody is stable for at least 2 years at 2 - 4?C.  After reconstitution, aliquot and store at -20°C or -80°C.  Avoid repeated freezing and thawing.
This is a Novozymes GroPep specification. ibt is authorized distributor of Novozymes GroPep!

 Datasheet for Anti-human/rat IGFBP-4, polyclonal antibody, rabbit