Product Code Specification:

The Product:  The IGFBP-3 IgG fraction was purified from a polyclonal antiserum obtained from rabbits immunized with a synthetic peptide of unique sequence from the central domain of human IGFBP-3. The antibody has been characterized for immunohistochemistry and EIA.

Quantity:  20 ug. Specificity:  In EIA the antibody does not cross-react with human IGFBP-1, -2, -4, -5 and –6.

Application: Characterized for the immunohistochemical detection of IGFBP-3 in OCT treated human liver sections and paraffin embedded sections of embryonic rat tissues. Titre:  A final dilution of 1/500 for IHC in embryonic rat tissues with biotinylated IgG secondary antibody and detection with ExtrAvidin conjugated Cy3. A final dilution of 1/50 – 1/100 for IHC in OCT treated human liver sections. Use at a final dilution of ~1/2,500 for EIA.

Reconstitution:  Dissolve in 400 µl sterile water (1/20 dilution).  Dilute further in a buffer solution appropriate for the desired application. Storage/Stability:  In lyophilized form the antibody is stable for at least 2 years at 2 - 4 °C.  After reconstitution, aliquot and store at -20 °C or -80 °C.  Avoid repeated freezing and thawing.

This is a GroPep product specification. ibt is authorized distributor for GroPep products.

 Datasheet for Anti-human/rat IGFBP-3, polyclonal antibody, rabbit