Product Specifcations Anti-human/mouse/rat IGFBP-6:

The Product:  The IGFBP-6 IgG fraction was purified from a polyclonal antiserum obtained from rabbits immunized with a synthetic peptide of unique sequence from the central domain of human IGFBP-6. The antibody has been characterized for immunohistochemistry and EIA.

Quantity:  20 ug.

Specificity:  In EIA the antibody does not cross-react with human IGFBPs 1-5.

Application: Characterized for the immunohistochemical detection of IGFBP-6 in paraffin embedded sections of embryonic rat tissues.

Titre: A final dilution of 1/500 for IHC with GroPep’s protocol using a biotinylated IgG secondary antibody and detection with ExtrAvidin conjugated Cy3. Use at a final dilution of ~1/3,000 for EIA.

Reconstitution:  Dissolve in 400 µl sterile water (1/20 dilution).  Dilute further in a buffer solution appropriate for the desired application.

Storage/Stability:  In lyophilized form the antibody is stable for at least 2 years at 2 - 4 °C.  After reconstitution, aliquot and store at -20 °C or -80°C.  Avoid repeated freezing and thawing.

Reference: Lipinski et. al.:Sonic hedgehog signaling regulates the expression of insulin-like growth factor binding protein-6 during fetal prostate development. Developmental Dynamics 2005,Volume 233, Issue 3, Pages 829-836.

Link to Article.

This is a Novozymes GroPep specification. ibt is authorized distributor of Novozymes GroPep!

 Datasheet for Anti-human/mouse/rat IGFBP-6, polyclonal antibody, rabbit