Product specifications monoclonal mouse  Anti-Nucleoprotein of Puumula Virus (PUUV):  

Clone: 1G3/H10.

Description: Mouse monoclonal antibody IgG fraction (clone 1G3/H10) obtained by immunizing mice with purified recombinant PUUV Nucleoprotein. The IgG fraction was purified using Protein G-Sepharose.

Isotype: IgG-1.

Storage: Store at -20ºC.

Stability: Stable at least one year at -20ºC. Avoid repeated freezing and thawing.

Applications: This antibody can be used in ELISA (1:50,000 dilution) and Western blot (1:20,000 dilution) analysis. It recognizes specifically the nucleoprotein of PUUV. Hantaviruses (family Bunyaviridae) are enveloped viruses that contain a tripartite, negative-stranded RNA genome encoding an RNA-dependent RNA polymerase (L), two integral membrane glycoproteins (G1 and G2), and a nucleocapsid (N) protein. The N protein is the most abundant viral component and the major antigen in early serological response in humans and mice. It has essential functions in viral RNA replication, encapsidation, and also in virus assembly. PUUV is a causative agent of a mild form of hemorrhagic fever with renal syndrome (nephropathia epidemica) mainly in Europe and it is transmitted by the red bank vole Clethrionomys glareolus.

Formulation: Solution at 1.0 mg/mL in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.

References:

Lindgren, L. et al. (2006) Virus Genes 33, 169-174.
Plyusnin, A. et al (2001) Adv. Virus Res. 57, 105-136.
Sironen, T. et al. (2001) J. Virol. 75, 11803-11810.
Stohwasser, R. et al. (1990) Virology 174, 79-86.

 Datasheet for Anti-Hantavirus Puumula Strain (PUUV) Nucleoprotein, monoclonal, mouse