Product specifications monoclonal mouse Anti-Glycoprotein 1 of Puumula virus (PUUV). IgG fraction:

Clone: 7C8/F12.

Description: Mouse monoclonal antibody IgG fraction (clone 7C8/F12) obtained by immunizing mice with purified recombinant PUUV Glycoprotein 1 (G1). The IgG fraction was purified using Protein G-Sepharose.

Isotype: IgG1.

Storage: Store at -20ºC.

Stability: Stable at least one year at -20ºC. Avoid repeated freezing and thawing.

Applications: This antibody can be used in ELISA (1:10,000 dilution) and Western blot (1:20,000 dilution) analysis. It recognizes G1 glycoprotein of PUUV and it has cross reactivity with Dobrava virus (DOBV), Puumula virus (PUUV), Sin Nombre virus (SNV) and Andes virus (ANDV). Hantaviruses (family Bunyaviridae) are enveloped viruses that contain a tripartite, negative-stranded RNA genome encoding an RNA-dependent RNA polymerase (L), two integral membrane glycoproteins (G1 and G2), and a nucleocapsid (N) protein. The G1 and G2 glycoproteins form heterodimers and they attach the virion to a cell receptor and to promote fusion membrane after endocytosis of the virion. G1 can dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis. PUUV is a causative agent of a mild form of hemorrhagic fever with renal syndrome (nephropathia epidemica) mainly in Europe and it is transmitted by the red bank vole Clethrionomys glareolus.

Formulation: Solution at 1.0 mg/ml in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.

References:   

Plyusnin, A. et al (2001) Adv. Virus Res. 57, 105-136.
Sironen, T. et al. (2001) J. Virol. 75, 11803-11810.
Giebel, L.B. et al. (1989) Virology 172, 498-505.

 Datasheet for Anti-Hantavirus Puumula Strain (PUUV) Glycoprotein 1, monoclonal, mouse