Product specifications monoclonal mouse Anti-Nucleoprotein of Hantaan Virus (HTNV):

Clone: 5H7/G5.

Description: Mouse monoclonal antibody IgG fraction (clone 5H7/G5) obtained by immunizing mice with purified recombinant HTNV Nucleoprotein. The IgG fraction was purified using Protein G-Sepharose.

Isotype: IgG-2b.

Storage: Store at -20ºC.

Stability: Stable at least one year at -20ºC. Avoid repeated freezing and thawing.

Applications: This antibody can be used in ELISA (1:1,000 dilution) and Western blot (1:8,000 dilution) analysis. It recognizes specifically the nucleoprotein of HTNV. Hantaviruses (family Bunyaviridae) are enveloped viruses that contain a tripartite, negative-stranded RNA genome encoding an RNA-dependent RNA polymerase (L), two integral membrane glycoproteins (G1 and G2), and a nucleocapsid (N) protein. The N protein is the most abundant viral component and the major antigen in early serological response in humans and mice. It has essential functions in viral RNA replication, encapsidation, and also in virus assembly. HTNV is a causative agent of severe hemorrhagic fever mainly in China and it is transmitted by the rodent Apodemus agrarius.

Formulation: Solution at 1.0 mg/ml in PBS. Some of the liquid in the vial could have evaporated with changes in the final volume. However, the mass of the protein is still inside the vial.

References:

Zou, Y. et al. (2008) J. Gen. Virol. 89, 1987-1997
Yan, L. et al (2007) Emerg. Infect. Dis. 13, 1301-1306-
Severson, W. E. et al. (2001) J. Virol. 75, 2646-2652.
Schmaljohn, C.S. et al. (1986) Virology 155, 633-643.

 Datasheet for Anti-Hantavirus Hantaan strain (HTNV) nucleoprotein, monoclonal, mouse